Question

Describe the role of CaCl2 in the preparation of competent cells?

Answer 1

Answer:

CaCl2 is known to increase the efficiency of DNA uptake to produce transformed bacterial cells. The divalent Ca2+ ions supposedly create transient pores on the bacterial cell wall by which the entry of foreign DNA is facilitated into the bacterial cells.

Explanation:

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Answer 2

Answer:

CaCl2 is known to increase the efficiency of DNA uptake to produce transformed bacterial cells. The divalent Ca2+ ions supposedly create transient pores on the bacterial cell wall by which the entry of foreign DNA is facilitated into the bacterial cells.