Question

Describe the roles of heat, primers and the bacterium Thermus aquaticus in the process of PCR.

Answer 1

Role of PCR technique

Explanation:

• The role of heat, primers, and the bacterium Thermus aquaticus during the procedure of PCR:  
• Heat: It denatures PCR. The ds DNA is warmed at the temperature 95 degrees Celsius, both the strands independent.  
• Primers: Primers are the little oligonucleotides of fluctuating lengths of around 10-18 nucleotides that are correlative to a locale of format DNA and aides in the expansion of another chain.  
• Thermus aquaticus: A specific thermostable chemical Taq DNA polymerase is confined from the bacterium Thermus aquaticus which can endure high temperatures and structures another strand.