Describe the steps of western blotting
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Western blotting is an important technique used in cell and molecular biology. By using a western blot, researchers are able to identify specific proteins from a complex mixture of proteins extracted from cells.
botanyliker:
step wise describe plz
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The standard steps in Western blotting.
Samples are prepared and loaded on to a gel and during the electrophoresis the negatively charged proteins move towards the positively charged anode.
In order to further analyze the protein,they are transferred on to a membrane in a procedure called blotting.
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