Describe the various steps involved in tissue culture.
Answers
Answer:
The steps are:
1. Inoculation of Explant
2. Incubation of Culture
3. Sub-Culturing
Step # 1. Inoculation of Explant:
Successful control of contamination largely depends upon the precautions taken to prevent the entry of microorganisms at the time of transferring the sterilised explants on the nutrient medium.
Step # 2. Incubation of Culture:
After inoculation, the cultures are incubated in culture room or in a BOD incubator at 25±2°C temp. For certain plant or for some particular culture type below or above 25°C is needed.
Step # 3. Sub-Culturing:
The growth and development of tissues cultured in vitro are generally monitored by observing the cultures at regular intervals in the culture room or incubators.
Step # 4. Transplantation of the Regenerated Plant:
Plants regenerated from in vitro tissue culture are transplanted to soil in pots. Prior to transfer to pots the acclimatization of these regenerated plants are needed.