Question

Describe the various steps involved in tissue culture.
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Answer 1

Answer:

The steps are:

1. Inoculation of Explant

2. Incubation of Culture

3. Sub-Culturing

Answer 2

Step # 1. Inoculation of Explant:

Successful control of contamination largely depends upon the precautions taken to prevent the entry of microorganisms at the time of transferring the sterilised explants on the nutrient medium.

Step # 2. Incubation of Culture:

After inoculation, the cultures are incubated in culture room or in a BOD incubator at 25±2°C temp. For certain plant or for some particular culture type below or above 25°C is needed.

Step # 3. Sub-Culturing:

The growth and development of tissues cultured in vitro are generally monitored by observing the cultures at regular intervals in the culture room or incubators.

Step # 4. Transplantation of the Regenerated Plant:

Plants regenerated from in vitro tissue culture are transplanted to soil in pots. Prior to transfer to pots the acclimatization of these regenerated plants are needed.