details of modern of diagnosis_??
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taking an appropriate history of symptoms and collecting relevant data.
physical examination.
generating a provisional and differential diagnosis.
testing (ordering, reviewing, and acting on test results)
reaching a final diagnosis.
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details of modern methods of diagnosis_??
Details of modern methods of diagnosis :
1. PCR
- It helps to detect very low concentration of bacteria or virus at the time when the symptoms of the disease are not visible by amplification of their nucleic acid.
- PCR is now routinely used to detect HIV in suspected AIDS patient.
- It is being used to detect mutations in genes in suspected cancer patients too.
- It is a powerful technique to identify many other genetic disorders.
2. ELISA
- Based on the principle of Antigen-Antibody interactions.
- Infection by pathogen can be detected by the presence of antigens (proteins, glycoproteins) or by detecting the antibodies synthesized against the pathogen.
- The enzymes frequently used in ELISA include peroxidase and alkaline phosphatase.
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