Physics, asked by rajharihar7573, 3 months ago

detecting reagent used to identify glycyrrhizic acid.​

Answers

Answered by Anonymous
0

Answer:

Abstract

Explanation:

The invention relates to a method for determining glycyrrhizic acid content in extract after polysaccharide extraction of glycyrrhiza by virtue of a vanillin-sulphuric acid. The method comprises the following steps of: (1) preparing a glycyrrhizic acid standard solution and a sample solution to be detected; preprocessing the glycyrrhizic acid standard solution and the sample solution to be detected; and  determining absorbency. According to the invention, an ethanol (v/v) solution with the concentration of 10%-30% is selected as a solvent according to the characteristic that glycyrrhiza extract does not contain saccharides, the solvent is subjected to a preprocessing step for removing the ethanol interference before solution color development, the vanillin-sulphuric acid is used as a color reagent, since the method is free from saccharide interference, the sensitivity can be further increased, and the operation is simpler and more convenient as only one color reagent is used. The solution develops color under an optimal color developing condition obtained in observation by using the vanillin-sulphuric acid as the color reagent after the solution is subjected to the preprocessing, the stability is good, the accuracy and the recovery rate are high, and the method can be better used for determining the variation trend of the glycyrrhizic acid content after the glycyrrhizic acid is extracted and separated from the extract after the polysaccharide is extracted completely from the glycyrrhiza.

Answered by nairaryaashok01
0

Answer:

The detecting reagent used to identify glycyrrhizic acid is vanilla-sulphuric acid.

Explanation:

What is glycyrrhizic acid?

  • It is a triterpene glycoside most commonly found in liquorice.
  • it can be separated by High fraction liquid chromatography.
  • The most efficient detecting agent used to identify glycyrrhizic acid is vanilla-sulphuric acid.
  • Vanilla-sulphuric acid is a chromogenic agent, it changes colour based on the quality of the glycoside.
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