Detection of active bacterial population in soil
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The analysis of natural microbial communities is limited
by reliance on growth-dependent methods for identifying
individual species at the phenotypic level. So far, two
techniques for specific enumeration of bacteria have
been established : selective plating and immunofluores-
cence (Hill & Gray, 1967; Bohlool & Schmidt, 1980;
Postma et al., 1988; Thompson et al., 1990). Both
techniques depend on the isolation of the target
organisms. While selective plating techniques rely on
strong phenotypic markers of the target organism, e.g.
antibiotic resistance, and their ability to grow on
selective media, the immunofluorescence technique
needs pure cultures of the target organism in orde
by reliance on growth-dependent methods for identifying
individual species at the phenotypic level. So far, two
techniques for specific enumeration of bacteria have
been established : selective plating and immunofluores-
cence (Hill & Gray, 1967; Bohlool & Schmidt, 1980;
Postma et al., 1988; Thompson et al., 1990). Both
techniques depend on the isolation of the target
organisms. While selective plating techniques rely on
strong phenotypic markers of the target organism, e.g.
antibiotic resistance, and their ability to grow on
selective media, the immunofluorescence technique
needs pure cultures of the target organism in orde
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