Determination of adenosine triphosphate and its breakdown products in fish muscle by high-performance liquid chromatography
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Adenosine mono-, di-, and triphosphates, inosine monophosphate, inosine, and hypoxanthine in neutralized perchloric acid extracts of fish muscle were resolved in a single run by high-performance liquid chromatography. Recoveries of reference compounds added to fish muscle/perchloric acid homogenates were 94-100% and responses to repeated injections of a mixed standard were highly reproducible. Analysis time was reduced to 20% of that for previous methods which use ion exchange chromatography. K values were computed directly from the results.
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Determination of adenosine triphosphate and its breakdown products in fish muscle by high- performance liquid chromatography.
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