Question

Determine chlorophyll content through spectrophotometer

Answer 1

Representations of s and p atomic orbitals. The valence bond theory states that atoms in a covalent bond share electron density through the overlapping of their valence atomic orbitals. This creates an area of electron pair density between the two atoms.

Answer 2

Chlorophyll content is obtained by rinsed in 85% acetone solution which is based on Mackinney's work and measuring its absorbance using Campspec M501 Single Beam UV/vis Spectrophotometer at λ = 663 nm and λ = 645 nm. Arnon formulated Mackinney's work to get chlorophyll concentration !