Question

Determine the yield of cell for per gram of glucose consumed

Answer 1

Let's consider the specific substrate consumption rate with these data:

TIME of inoculation (0 h)

9.66g of substrate present in the medium

inoculum corresponds to around 0.25 gDW

TIME 13.25 h

0.94 g of substrate left in the medium

biomass concentration is 5.3 gDW

First of all I would like to know if the formula I'm using is correct:

qs = g of substrate consumed / (time * g DryWeight)

Here's how I do it:

qs = (9.66 g - 0.94 g) / (13.25 h * 5.3gDW) = 8.72 / 70.2 = 0.124 g g-1 h-1