Question

Determintion of reaction rateby fluorescence

Answer 1

In analytical kinetic methods, the change in concentration of some species with time is measured and related to the original analyte concentration. A dynamic measurement may be made during the reaction in which the rate of change of the monitored characteristic is obtained, or the reaction may be quenched after a given time period and a static measurement made. A suitable reaction monitoring technique such as spectrophotometry, potentiometry, fluorometry, or amperometry can be employed. The measured change in reaction monitor signal is related to the initial concentration of the analyte. This type of analytical measurement scheme can be contrasted with more conventional equilibrium-based procedures in which the analytical reaction is allowed to go to completion before measurements are made of the reaction monitor signal.