Deyermination.Of viable count of cells tissue culture using trypan blue
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The Trypan Blue dye exclusion test is used to determine the number of viable cells present in a cell suspension. ... Periodic cell viability assessment provides an early indicator of the quality of your fresh cells prior to freezing. Viabilities of greater than or equal to 95% are excellent.
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Cell densities are commonly measured by manually counting dilutions of a culture in the presence of a staining agent like trypan blue.
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