Question

Diagramatically represent the expeimantal steps in cloning and expressing an human gene ( say the gene for growth hormone) into a bacterium like Ecoli?

Answer 1

DNA cloning is a method of producing multiple identical copies of specific template DNA. It involves the use of a vector to carry the specific foreign DNA fragment into the host cell. The mechanism of cloning and transfer of gene for growth hormone intoE.coli is represented below

Answer 2

Using the recombinant DNA technology it is possible to isolate and clone single copy of a gene or a DNA segment into an indefinite number of identical copies. So that the inserted DNA also replicates faithfully with the parent DNA is called as gene cloning.

Explanation:

Diagram is attached to show  experimental steps in cloning and expressing an human gene ( say the gene for growth hormone) into a bacterium like Ecoli.

1. A fragment of DNA to be cloned is first inserted into a plasmid vector containing an amipicillin-resistance gene(amp), shown in given figure.
2. Only the few cells transformed by incorporation of a plasmid molecule will survive on ampicillin-containing medium.
3. In transformed cells, the plasmid DNA replicates and segregates into daughter cells, resulting in formation of an ampicillin-resistant colony.