Question

Dideoxynucleoside triphosphates (ddntps) are used in sequencing dna, because

Answer 1

DNA sequencing is a laboratory method used to determine the sequence of a DNA molecule. The method was developed by Frederick Sanger in 1975, who was later awarded the Nobel Prize in chemistryin 1980 for his contributions to understanding DNA sequences. Consequently, it is often referred to as Sanger Sequencing.In Sanger sequencing, the DNA to be sequenced serves as a template for DNA synthesis. A DNA primer is designed to be a starting point for DNA synthesis on the strand of DNA to be sequenced. Four individual DNAsynthesis reactions are performed. The four reactions include normal A, G, C, and T deoxynucleotide triphosphates (dNTPs), and each contains a low level of one of four dideoxynucleotide triphosphates (ddNTPs): ddATP, ddGTP, ddCTP, or ddTTP. The four reactions can be named A, G, C and T, according to which of the four ddNTPs was included. When a ddNTP is incorporated into a chain of nucleotides, synthesis terminates.

Answer 2

ANSWER
.........
1. IT IS THE LABROTARY METHOD WHICH IS USED TO DETERMINE THE SEQUENCE OF THE DNA MOLECULES.

2. IT IS FIRSTLY USED IN 1975