Question

Difference between aceto orcein stain and lacto ace to orcein stain?

Answer 1

Acetocarmine preparation (1% solution) Carmine is a basic dye that is prepared from the insect Coccus cacti. Dissolve 10 g carmine (Fisher C579-25) in 1 L of 45% glacial acetic acid, add boileezers, and reflux for 24 h. Filter into dark bottles and store at 4°C.Aceto-orcein staining does not require the addition of iron ions. The staining prodedure is similar to the aceto-carmine method. Fixed material is transferred for an approprioate time to 1% aceto-orcein and then analyzed by the squash technique.

Answer 2

Answer:

Orcein is also used as a stain in microscopy to visualize chromosomes, elastic fibers, Hepatitis B surface antigens, and copper-associated proteins.