Difference between Biosafety level 3 and 4
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Difference between Biosefety level 3 and4 are
Standard personal protective equipment must be worn, and respirators might be required
Solid-front wraparound gowns, scrub suits or coveralls are often required
All work with microbes must be performed within an appropriate BSC
Access hands-free sink and eyewash are available near the exit
Sustained directional airflow to draw air into the laboratory from clean areas towards potentially contaminated areas (Exhaust air cannot be re-circulated)
A self closing set of locking doors with access away from general building corridors
Access to a BSL-3 laboratory is restricted and controlled at all times.
In addition to BSL-3 considerations, BSL-4 laboratories have the following containment requirements:
Personnel are required to change clothing before entering, shower upon exiting
Decontamination of all materials before exiting
Personnel must wear appropriate personal protective equipment from prior BSL levels, as well as a full body, air-supplied, positive pressure suit
A Class III biological safety cabinet