Question

Difference between brightfield and darkfield microscopy

Answer 1

Brightfield microscopy is the conventional technique. It is suitable for observing the natural colors of a specimen or the observation of stained samples. The specimen appears darker on a bright background.

Darkfield microscopy shows the specimens bright on a dark background. Brightfield microscopes that have a condenser with a filter holder can be easily converted to darkfield by placing a patch stop filter into the filter holder. The specimens appear bright, because they reflect the light from the microscope into the objective.

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