Question

difference between cell culture and protoplast culture​

Answer 1

Answer:

Protoplast Culture: definition Isolated protoplasts have been described as "naked" cells because the cell wall has been removed by either a mechanical or an enzymatic process. In the isolated protoplast the outer plasma membrane is fully exposed.

☝️Only the definition...

Answer: Single-cell culture: the growing of individual cells which have been obtained from a tissue, callus or suspension culture, with the aid of enzymes or mechanically. ... Protoplast culture: the culture of protoplasts obtained from cells by enzymatic digestion of the cell wall.

Answer 2

Cell culture :

• Cell culture is the process of growing cells under controlled conditions, usually outside their natural environment.
• Once the cells of interest have been isolated from living tissue, they can be maintained under carefully controlled conditions.
• Growth of microorganisms such as bacteria, yeast, human, plant and animal cells in the laboratory.
• Cell cultures can be used for infectious disease diagnosis, new drug testing, and research.
• Cells cultured in the laboratory can be divided into three types: primary cells, transformed cells, and self-renewing cells.

Protoplast culture:

• In protoplast culture, protoplasts isolated from any part of the plant, including roots, shoots, leaves, or embryos, are cultured in artificial media under artificial conditions that favor cell division and plant regeneration.
• The steps involve,culture of protoplasts to grow whole plants, cell fusion, and introduction of foreign genetic material into protoplasts.
• There are various methods of protoplast culture, including liquid culture, agar culture, droplet culture, co-culture, hanging droplet culture, immobilized/bead culture, and feeder layer method.

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