Question

Difference between direct and indirect elisa

Answer 1

Hi friend here is ur answer :--

• Direct Elisa —> In indirect Elisa only the antibody is used — the single antibody is conjugated directly to the detection enyme.
• Indirect elisa —> The indirect Elisa requires two antibodies — a primary antibody and an enzyme like secondary antibody that is complementary to the primary antibody.

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Answer 2

Answer:

Direct ELISAs use a conjugated primary antibody, while indirect ELISAs include an additional amplification step. In an indirect ELISA, an unconjugated primary antibody binds to the antigen, then a labeled secondary antibody directed against the host species of the primary antibody binds to the primary antibody.