Difference between gel electrophoresis and pcr
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Gel electrophoresis :
It is the process used in restriction enzyme digestion.In this process the DNA fragments are placed on it.As the DNA fragments are negatively charged they move towards positively charged anode through agarose medium. The fragments can be separated based on their size because smaller fragments move farther than larger ones.The DNA bands can be seen by staining them with ethidium bromide and exposing to UV light..
PCR :
PCR means polymerase chain reaction . In this process multiple copies of a single gene can be produced.It requires Taq polymerase and deoxy nucleoside triphosphates in an instrument called Thermocycler. Nowadays PCR is majorly used technique and so it is also called as people's chain reaction.
It is the process used in restriction enzyme digestion.In this process the DNA fragments are placed on it.As the DNA fragments are negatively charged they move towards positively charged anode through agarose medium. The fragments can be separated based on their size because smaller fragments move farther than larger ones.The DNA bands can be seen by staining them with ethidium bromide and exposing to UV light..
PCR :
PCR means polymerase chain reaction . In this process multiple copies of a single gene can be produced.It requires Taq polymerase and deoxy nucleoside triphosphates in an instrument called Thermocycler. Nowadays PCR is majorly used technique and so it is also called as people's chain reaction.
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Explanation:
The results of a PCR reaction are usually visualized (made visible) using gel electrophoresis. Gel electrophoresis is a technique in which fragments of DNA are pulled through a gel matrix by an electric current, and it separates DNA fragments according to size. ... Right lane: result of PCR reaction, a band at 400 bp.
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