Question

difference between gram positive and gram negative stain.[in points]

Answer 1

Characteristics Gram Positive Gram Negative

1. Gram Reaction Retain crystal violet dye and stain blue or purple.

Gram-Positive

Accept safranin and stain pink or red

Gram-Negative

2. Cell Wall Structure Structure of Gram Positive cell wall :

gram-positive-cell-wall Structure of Gram Negative cell wall :

gram-negative-cell-wall

3. Cell Wall Thickness Thick (20-80 nm) Thin (8-10 nm)

4. Chemical Composition of Cell Wall Peptidoglycan, Teichoic acid Lipotechoic acid Lipopolysaccharide, Lipoproteins and Peptidoglycans

5. Peptidoglycan Layer Thick (Multilayered) Thin (Single layered)

6. Teichoic Acid in Cell Wall Present Absent