Difference between inverted and upright microscopy
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1) An inverted microscope gives you greater freedom than an upright one. For upright microscopes, the size of the sample is limited to an average height of 80 mm and a weight of 3 kg, depending also on the objective that is being used. ... Because the optics are below the stage, the sample is placed above the objectives
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In the Upright microscopes, the objectives are placed on top of the stage. Such microscopes are good to study fixed samples or the samples squeezed between the slide and the coverslip. On the other hand, in the Inverted Microscopes, the objectives are placed below the stage. These are widely used for live cell imaging.
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