Difference between nitrocellulose filter & nitrocellulose membrane
Answers
What is a Nitrocellulose Membrane?
Nitrocellulose membrane is a commonly used membrane filter in nucleic acid and protein blotting techniques. It has a high protein binding potential. Hence, nitrocellulose membranes are widely used in western blotting technique. Nitrocellulose membranes are compatible with all hybridization techniques and show superior binding capacities without interferences. Nitrocellulose membranes are naturally hydrophilic in nature. They make hydrophilic interaction with molecules and efficiently immobilize them onto the membrane. Commercial nitrocellulose membranes are available in two pore sizes: 0.45 and 0.2 µm.
Key Difference - Nitrocellulose vs Nylon Membrane
Figure 01: Nitrocellulose membrane used in western blotting
What is a Nylon Membrane?
Nylon membrane is another type of commercial membrane used in blotting techniques. It is alternatively used with nitrocellulose membranes for southern and northern blotting. Nylon membranes are ideal for southern blotting than nitrocellulose due to their high affinity to binding with DNA. Due to several unique features of nylon membranes, researchers commonly use nylon membranes for southern and northern blotting instead of over nitrocellulose membrane. Nylon membranes are also recommended for stripping and reprobing, unlike nitrocellulose
Answer:
Explanation:
Both are same only