Difference between preparative and analytical centrifugation
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Centrifugation is a separation method in which the application of the centrifugal force sediments or fractionates a heterogeneous mixture. Microcentrifugation, high-velocity centrifugation, and ultracentrifugation are the three types of centrifugation based on the volume and speed used in the process. Among other centrifugation methods, ultracentrifugation uses the maximum angular velocity. The rotating speed of ultracentrifugation is as high as 1 000 000 g. Thus, ultracentrifugation is used to isolate small particles such as ribosomes, proteins, and viruses. Preparative and analytical centrifugation are the two types of ultracentrifugation methods. The main difference between preparative and analytical centrifugation is that preparative centrifugation is used in pelleting small materials such as membranes, organelles, viruses, DNA and RNAwhereas analytical centrifugation is used to determine the mass and shape of macromolecules such as protein complexes and rate of sedimentation of molecules
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