Difference between protonation and deprotonation
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Protonation is the addition of a proton to a chemical species. Deprotonation is the removal of a proton from a chemical compound.
The main difference between protonation and deprotonation is that protonation adds a +1 charge to a compound whereas deprotonation removes a +1 charge from a chemical compound.
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At a pH below the pKa for each functional group on the amino acid, the functional group is protonated. At a pH above the pKa for the functional group it is deprotonated. If the pH equals the pKa, the functional group is 50% protonated and 50% deprotonated
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