Question

Difference between recombinant cells from non-recombinants on the basis of their ability to produce colour in presence of a chromogenic substance. In this process an enzyme gene become inactivate which is reffered as -
(1) Insertional inactivation
(2) Insertional activation
(3) Chromosomal abberation
(4) Inverson

Answer 1

Answer:

hello ,........

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The recombinants do not produce any colour while, the non-recombinants produce a blue colour with chromogenic substrate in the medium.

(ii) The enzyme a-galactosidase become inactivated on insertion of recombinant DNA, within the coding sequence of enzyme.

answer of 2nd que. will be INSERTIONAL INACTIVATION .....AS........

Insertional inactivation is a technique used in recombinant DNA technology.

In this procedure, a bacteria carrying recombinant plasmids or a fragment of foreign DNA is made to insert into a restriction site inside a gene to resist antibiotics, hence causing the gene to turn non-functional or in an inactivated state.

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Answer 2

Answer:

The recombinants do not produce any colour while, the non-recombinants produce a blue colour with chromogenic substrate in the medium. (ii) The enzyme a-galactosidase become inactivated on insertion of recombinant DNA, within the coding sequence of enzyme.

Explanation:

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