Question

Difference between southern blot northern blot and western blot hybridization

Answer 1

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Southern Blotting

Western Blotting

Technique for the identification of specific DNA in a sample

Technique for the identification of specific protein in a sample

Developed by E.M. Southern therefore called “ Southern”

The term “Western” has no scientific significance just a misnomer to match with Southern blotting

Principle is Hybridization; the process of forming a double-stranded DNA molecule between a single-stranded DNA probe and a single-stranded target DNA. (Southern Blotting Steps)

Principle: Antigen-antibody interaction, an immunodetection method

(Refer: Western Blotting)

Probe used is single stranded DNA or sometimes RNA

Probe used is antibodies specifically targeted against epitopes of antigens

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