Difference between split gene and overlapping genes
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Generally, the different genes occur as discrete units separate from each other, but some prokaryotic genes may be overlapping. Virtually all the prokaryotic genes are functional units. But eukaryotic genes present some interesting organisations such as split genes, overlapping genes, pseudogenes, etc.
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As it can replicate within a bacterial cell, it is used as a vector in r-DNA technology. Production of insulin polypeptide chains separately by plasmids of E.coli have enables artificial production of mature human insulin.They are extracted and combined by creating disulphide bridge to form human insulin.
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