Question

Difference between thin layer chromatography and column chromatography

Answer 1

This difference of stationary phase results in numerous advantages for TLC , as compared to PC, namely:

1) Higher sample throughput and lower analysis time, as the number of samples that can be separated on one plate in TLC, is far more than the number of samples that can be separated in one paper.

2)Strong reagents can be used for the purpose of identification, as the TLC plate is able to withstand strong solvents and colour-forming agents.

3)TLC plates can be heated to higher temperatures(for spot development) without any damage, which might not be possible in case of PC, lest the paper gets damaged.

4)The sensitivity of detection is more in TLC, as compared to PC, because of lesser spreading of substance zones, in a TLC plate, than what occurs in PC.

Answer 2

"Chromatography is an advance separation method used to get the precise results. It is mainly used for separation of dyes and amino acids.

Considering stationary and mobile phase, it can be classified as "thin layer chromatography" and "column chromatography".

The main "difference between" these two is that as "thin layer chromatography" uses a different stationary phase than column chromatography.

Another difference is that "thin layer chromatography" can be used to distinguish non-volatile mixtures which is not possible in column chromatography."