Question

Difference in conformation for separation of plasmid and chromosomal dna

Answer 1

When working with RNA, care must be taken to avoid degradation by RNases, which are extremely stable and active. ... Beta-mercaptoethanol (ß-ME) is a reducing agent that will irreversibly denature RNases by reducing disulfide bonds and destroying the native conformation required for enzyme functionality.

Answer 2

Hi,
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Here is your answer,
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When working with RNA care must be taken to avoid degraded by RNases, which are extremely stable and active, Beta marcoptoethonol, is a reducing agent they will inversely donate RNases by reducing disulfide bads and destroy the native conformation requite for enzyme.
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