differentiate between 20 gel electrophoresis & IEF?
Answers
Answer:
Explanation:
Isoelectric focusing (IEF) separates proteins on the basis of their isoelectric point. It can also be used as the first dimension of 2-D gel electrophoresis.
PAGE can be divided into native and SDS-PAGE. Native PAGE separates proteins that have not been denatured based on a combination of mass and charge. SDS-PAGE separates denatured proteins on the basis of mass only. SDS-PAGE can also be used as the second dimension of 2-D gel electrophoresis, and is also the starting point for Western blotting.
Answer: Two-dimensional (2D) PAGE separates proteins by native isoelectric point in the first dimension and by mass in the second dimension. SDS-PAGE separates proteins primarily by mass because the ionic detergent SDS denatures and binds to proteins to make them uniformly negatively charged.
Explanation: