diffrence between promoter and terminator in genes and genomes
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This assay measures the distance between an end label (at a specific known site on DNA) and the end of a duplex between RNA and the labeled DNA. A fragment of DNA (complementary to the RNA) that extends beyond the 5' end of the RNA is labeled at a restriction site within the RNA‑complementary region. The labeled DNA is hybridized to RNA and then digested with the single‑strand specific nuclease S1. The resulting fragment of protected DNA is run on a denaturing gel to determine its size. Note that this fragment runs from the labeled site to the nearest interruption between the DNA and the RNA. This could be the beginning of the RNA, or it could be an intron, or it could be an S1 sensitive site.
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