Question

Dimminution of outer membrane permeability by mg2+ in a marine pseudomonad

Answer 1

Explanation:

Abstract. Intact cells of the marine pseudomonad MB-45 , in the presence of optimal Mg2+, exhibited little alkaline phosphatase activity as judged by the hydrolysis

Answer 2

Answer:

Adding Mg2+ to the released enzyme restored its activity to that of the cell-bound form. The synthesis of alkaline phosphatase by the cells was not affected by adding 50 mM inorganic phosphate to the growth medium. The Km of the released enzyme for p-nitrophenyl phosphate was found to be 6.1 × 10−5 M.