Chemistry, asked by Avaneesh2425, 1 year ago

disadvantage of paper chromatography

Answers

Answered by Anonymous
2
1. Paper chromatographic techniques can not be used in separation of volatile substances such as hydrocarbons and volatile fatty acids.

2. The lower limit for the detection of most compounds is 1-5 microgram.
Answered by ShivPriya
7
Heya !!!

DISADVANTAGES

■Complex mixture cannot be separated by paper chromatography .

■Less accurate compared to HPLC .

■In quantitative analysis paper chromatography is not effective .

■Large quantity of sample cannot be applied on paper chromatography.

■Paper chromatographic techniques can not be used in separation of volatile substances such as hydrocarbons and volatile fatty acids .

■ The lower limit for the detection of most compounds is 1-5 microgram .

■Accidently dipping sample spot in the solvent entails to repetition of the entire procedure .

hope it helps u dear ^_^
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