Disadvantages of immobilization
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Immobilistaion requires additional time, equipment and materials so is more expensive to set up.
Immobilised enzymes may be less active as they can not mix freely with the substrate.
Any contamination is costly to deal with because the whole system would need to be stopped.
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The disadvantages, however, include exposure of enzymes to microbial attack, physical abrasion of enzyme due to turbulence associated with the bulk solution. The major disadvantage of the internal immobilization method is the pore diffusion.
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