discuss different methods of collection of csf and body fluids
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produced at the choroid plexus of the 4 ventricles by modified ependymal cells
1. at rate of 20ml/hr in adults.
1. at rate of 20ml/hr in adults.
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A lumbar puncture, or “spinal tap,” is used to collect CSF for culture. A physician will insert a needle into the space between two vertebrae in the lower spine. The needle will then be moved carefully into the CSF-filled space surrounding the spinal cord. When the needle is in place, fluid can drip out into a collection vial. More than one vial may be needed, and the procedure can take several minutes.
Body Fluid Collection::::
All body fluids must be taken to the laboratory immediately after collection. Hand the specimen directly to the laboratory personnel (do not leave on counter). Note: cell lysis can begin within one hour of collection.Fluid stability is unknown and samples must be tested as soon as possible. Serous fluid (pleural, peritoneal, pericardial) may contain debris from the collection of the specimen. It is essential to put Hematology samples into EDTA immediately. For Chemistry samples, please see specific requirements in the chart below.Fluid collection for Crystals must follow requirements as indicated in the chart below.If unable to collect optimum volume of fluid refer to the Alphabetical Test Directory for specific test volumes. Samples may be shared for certain tests; contact the laboratory for assistance. ALWAYS indicate priority of test orders on the requisition.State source of fluid on requisition. If samples are collected from more than one site, each specimen and requisition must be labelled with the source. Ensure specimens are labelled with two identifiers.Clotting of sample will occur when fibrinogen is introduced with a traumatic puncture.Pleural fluid pH must be collected in a blood gas syringe. The sample must not be exposed to air (i.e. collect anaerobically and expel all bubbles). DO NOT SEND OTHER FLUIDS IN SYRINGES (WITH OR WITHOUT NEEDLES ATTACHED). DO NOT SEND FLUIDS IN TRANSFUSION BAGS, OR I.V. BOTTLES FOR HEMATOLOGY OR CHEMISTRY ANALYSIS.DO NOT SEND LARGE VOLUMES OF FLUID. POUR INTO APPROPRIATE CONTAINERS OR CONTACT LABORATORY FOR DIRECTION.
A lumbar puncture, or “spinal tap,” is used to collect CSF for culture. A physician will insert a needle into the space between two vertebrae in the lower spine. The needle will then be moved carefully into the CSF-filled space surrounding the spinal cord. When the needle is in place, fluid can drip out into a collection vial. More than one vial may be needed, and the procedure can take several minutes.
Body Fluid Collection::::
All body fluids must be taken to the laboratory immediately after collection. Hand the specimen directly to the laboratory personnel (do not leave on counter). Note: cell lysis can begin within one hour of collection.Fluid stability is unknown and samples must be tested as soon as possible. Serous fluid (pleural, peritoneal, pericardial) may contain debris from the collection of the specimen. It is essential to put Hematology samples into EDTA immediately. For Chemistry samples, please see specific requirements in the chart below.Fluid collection for Crystals must follow requirements as indicated in the chart below.If unable to collect optimum volume of fluid refer to the Alphabetical Test Directory for specific test volumes. Samples may be shared for certain tests; contact the laboratory for assistance. ALWAYS indicate priority of test orders on the requisition.State source of fluid on requisition. If samples are collected from more than one site, each specimen and requisition must be labelled with the source. Ensure specimens are labelled with two identifiers.Clotting of sample will occur when fibrinogen is introduced with a traumatic puncture.Pleural fluid pH must be collected in a blood gas syringe. The sample must not be exposed to air (i.e. collect anaerobically and expel all bubbles). DO NOT SEND OTHER FLUIDS IN SYRINGES (WITH OR WITHOUT NEEDLES ATTACHED). DO NOT SEND FLUIDS IN TRANSFUSION BAGS, OR I.V. BOTTLES FOR HEMATOLOGY OR CHEMISTRY ANALYSIS.DO NOT SEND LARGE VOLUMES OF FLUID. POUR INTO APPROPRIATE CONTAINERS OR CONTACT LABORATORY FOR DIRECTION.
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