Discuss in details 2D gel electrophoresis w.r.t. principle, instrumentation and applications.
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Detection of circular telomeric DNA without 2D gel electrophoresis. .... Application of this method to the soluble fraction of muscle tissues reliably resolves ~1800 ...... A summary of the identified proteins and their putative functions is discussed. ...... Specifically, we illustrate the working principle of the hybrid device, develop .
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In 2D GE proteins are separated as per isoelectric point and protein mass. Separation of the proteins by isoelectric point is called isoelectric focusing (IEF). When a gradient of pH is applied to a gel and an electric potential is applied across the gel, making one end more positive than the other.
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