discuss the role of selectable marker in vector during the experiment of biotechnology. explain with example
Answers
A selectable marker is a gene introduced into a cell, especially a bacterium or to cells in culture, that confers a trait suitable for artificial selection. They are a type of reporter gene used in laboratory microbiology, molecular biology, and genetic engineering to indicate the success of a transfection or other procedure meant to introduce foreign DNA into a cell. Selectable markers are often antibiotic resistance genes (An antibiotic resistance marker is a gene that produces a protein that provides cells expressing this protein with resistance to an antibiotic.). Bacteria that have been subjected to a procedure to introduce foreign DNA are grown on a medium containing an antibiotic, and those bacterial colonies that can grow have
successfully taken up and expressed the introduced genetic material. Normally the genes encoding resistance to antibiotics such as ampicillin, chloroamphenicol, tetracycline or kanamycin, etc., are considered useful selectable markers for E. coli.
Answer:
selectable markers are used to know weather desired gene is introduced in to recipient organism.
Explanation:
Ti gene is used as marker in transfer of Bt gene or nif gene.
If cotton develops gall then we say that marker ad desired Bt gene is introduced in genome of Cotton.