Question

Discuss the significance of colony counting and membrane filtration in microbial growth measurement.

Answer 1

The purpose of plate counting is to estimate the number of cells present based on their ability to give rise to colonies under specific conditions of nutrient medium, temperature and time

This is because the counting of CFU assumes that every colony is separate and founded by a single viable microbial cell.

Membrane filter technique is an effective, accepted technique for testing fluid samples for microbiological contamination. It involves less preparation than many traditional methods, and is one of a few methods that will allow the isolation and enumeration of microorganisms. Membrane filters are used extensively in the laboratory and in industry to sterilize fluid materials.

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