Discussion of separation of mononuclear cells by histopaque
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Blood is made up of a suspension of special cells in liquid called plasma. A lymphocyte is small white blood cells, usually 7 to 8 micrometers in length present in the blood. They are the only blood cells which can be transformed into actively proliferating cells. Lymphocytes are originated from lymphatic tissue throughout the body. This lymphocytes collected from blood have been in frequent use in the field of immunology. It helps to protect the body from diseases, invasion of foreign bodies, tumors and infections. For example, lymphocytes are used for the for immunological studies directed to histocompatibility antigens and cellular immunity. Lymphocytes are also used for determining lymphocyte blastotransformation or for determining the proportion of T cells to B cells, and for trying to separate helper T cells and Suppressor T cells from T cells.