Discussion on isolation of genomic dna from animal tissue
Answers
Answer:
A simple technique for the isolation of very high molecular weight genomic DNA from animal tissues and cells is described. The method involves rapid isolation of nuclei and their embedding in agarose beads followed by extraction of lipids and proteins with SDS. The protocol does not require proteolytic digestion and the whole procedure can be completed in 1 day. The isolated DNA is digestible by restriction enzymes and free of ligase inhibitors.
Explanation:
Two reagent based methods for extracting DNA are chelex and TRIzol. Using TRIzol, DNA will be in the bottom layer of your phenol/chloroform separation, although it can be problematic with phenol carry-over and DNA quality. Depending on your down stream application and sample size, I would use a commercially available kit. Qiagen has an excellent range but other companies supply cheaper alternatives.