distinguish between group 1 and group 2 self splicing
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Group I: Gunanine nucleoside cofactor is used whose 3′- OH makes a a nucleophilic attack on the phosphor and forms, 3′,5′- phosphodiester bond. ... Group II: 2′- OH group within the intron makes a nucleophilic attack on the 5′- splice site and forms a lariat like structure.
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Group I: Gunanine nucleoside cofactor is used whose 3′- OH makes a a nucleophilic attack on the phosphor and forms, 3′,5′- phosphodiester bond. ... Group II: 2′- OH group within the intron makes a nucleophilic attack on the 5′- splice site and forms a lariat like structure.
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