Question

Distinguish between sticky end and blunt end

Answer 1

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Answer: Blunt ends may result from the breaking of double-stranded DNA; however, there are no overhangs or unpaired bases. Longer overhangs are called cohesive ends or sticky ends. They are most often created by restriction endonucleases when they cut DNA.

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Answer 2

The ends in this case refer to the ends of the DNA strands. Depending on where and how the restriction enzyme cuts, it will produce either sticky ends or blunt ends. Sticky ends get their name because they have overlaps that allow the two ends to base-pair and join together with another DNA strand...

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