distinguish between thin layer chromatography and paper chromatography
Answers
Answer:
Thin Layer Chromatography is a technique used to isolate non-volatile mixtures. The experiment is conducted on a sheet of aluminium foil, plastic, or glass which is coated with a thin layer of adsorbent material. The material usually used is aluminium oxide, cellulose, or silica gel.
On completion of the separation, each component appears as spots separated vertically. Each spot has a retention factor (Rf) expressed as:
Rf = dist. travelled by sample / dist. travelled by solvent
The factors affecting retardation factor are the solvent system, amount of material spotted, absorbent and temperature. TLC is one of the fastest, least expensive, simplest and easiest chromatography technique.
Paper chromatography is one of the types of chromatography procedures which runs on a piece of specialized paper. It is a planar chromatography system wherein a cellulose filter paper acts as a stationary phase on which the separation of compounds occurs.
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Answer:
Explanation:
Thin Layer Chromatography is a technique used to isolate non-volatile mixtures. The experiment is conducted on a sheet of aluminium foil, plastic, or glass which is coated with a thin layer of adsorbent material. The material usually used is aluminium oxide, cellulose, or silica gel.
On completion of the separation, each component appears as spots separated vertically. Each spot has a retention factor (Rf) expressed as:
Rf = dist. travelled by sample / dist. travelled by solvent
The factors affecting retardation factor are the solvent system, amount of material spotted, absorbent and temperature. TLC is one of the fastest, least expensive, simplest and easiest chromatography technique.
Paper chromatography is one of the types of chromatography procedures which runs on a piece of specialized paper. It is a planar chromatography system wherein a cellulose filter paper acts as a stationary phase on which the separation of compounds occurs.