DNA replication enzymes are given below. Select their correct
sequence in DNA replication.
I. Helicase
II. SSB
III. Primase
IV. DNA polymerase
V. DNA ligase
(A) I➡️ V → IV → III → II (B) I ➡️II➡️ III➡️ IV ➡️ V
(C) I ➡️III ➡️II → IV → V (D) I➡️ IV➡️ III ➡️II → V
Answers
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Answer:
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Option B is correct
Explanation:
- DNA replication meaning synthesis of DNA that occur in three steps: Initiation, elongation, termination
- in the initiation step, two strands of DNA are separated so that they act as a template for the synthesis of new strand. this strand separation is done by DNA helicase
- in the next step to prevent reannealing single strand binding protein(SSB) binds to each template strand
- in the next step primase synthesize primer where DNA polymerase can add the new nucleotides.
- The next step is elongation: where DNA polymerase elongate the new strand by adding nucleotides.
- The lagging strand of the DNA is not synthesized as intact strand. several Okazaki fragments has generated. so, the ligase ultimately act on Okazaki fragments to ligate them into a single DNA strand
- so, the option B is correct
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