Question

Do restriction enzymes cut both strands of a double stranded DNA molecule?

Answer 1

DNA consists of two complementarystrands of nucleotides that spiral around each other in a double helix. Restriction enzymes cut through both nucleotidestrands, breaking the DNA into fragments, but they don't always do this in the same way.

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Answer 2

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Yeah..!!

~ restriction enzymes cut both strands of a double stranded DNA molecule during DNA replication.