Docking of protein-protein complex by had dock and molecular dynamics of complexes using gromacs
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Co-Immunoprecipitation (Co-IP) and Pull-Down
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The pull-down assay is a robust method by which a target protein is extracted from a mixture (e.g., cell lysate or serum) via its affinity to a specially prepared solid support. It is often possible to detect macromolecules that form stable complexes with the target protein in a pull-down assay—when the pull-down is accomplished using immobilized antibody, the method is known as coimmunoprecipitation (co-IP). After co-IP, immobilized immune complexes can be collected, eluted from the support, and identified, thereby revealing the composition of the retrieved multimers.
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The pull-down assay is a robust method by which a target protein is extracted from a mixture (e.g., cell lysate or serum) via its affinity to a specially prepared solid support. It is often possible to detect macromolecules that form stable complexes with the target protein in a pull-down assay—when the pull-down is accomplished using immobilized antibody, the method is known as coimmunoprecipitation (co-IP). After co-IP, immobilized immune complexes can be collected, eluted from the support, and identified, thereby revealing the composition of the retrieved multimers.
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