Question

does all enzymes exhibit optical activity at single PH and temperature​

Answer 1

Raman Optical Activity, Theory

Laurence A. Nafie, in Encyclopedia of Spectroscopy and Spectrometry, 1999

Raman optical activity (ROA) is defined as the difference in Raman scattering intensity for right minus left circularly polarized light. Along with optical rotational and circular dichroism, ROA is a form of natural optical activity. All forms of optical activity can be defined as the differential interaction of a molecule with right versus left circularly polarized radiation. Only chiral molecules exhibit natural optical activity and, for such molecules, the mirror image of the molecule cannot be superimposed on itself. The most common form of ROA is vibrational ROA. Vibrational ROA is also one of two form of vibrational optical activity. The other form is vibrational circular dichroism (VCD), which is the difference in the IR absorption of a molecule for left versus right circularly polarized radiation for a vibrational transition. VCD and ROA are complementary, non-redundant forms of vibrational optical activity in the same way that IR absorption and Raman scattering are complementary forms of ordinary vibrational spectroscopy.