Draw a schematic sketch of pBR 322 plasmid and label the following in it a)Any 2 restriction sites b) Ori and rop genes c) An antibiotic resistant gene.
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a) Pst I , Sal I , etc are the restriction sites of pBR 322.
b) ORI and ROP gene are labelled in the diagram.
c) Ampicillin resistance and Tetracycline resistance are the two antibiotic resistant gene.
EXTRA INFORMATION
ORI
- Full form if ORI is origin of replication.
- ORI is responsible for replication and it also controls the copy number.
Restriction sites
- These are also known as cloning sites.
- Vector can have many sites of restriction enzyme but only one site for each enzyme should be present.
Restriction enzymes
- It is a type of endonuclease enzyme.
- These enzymes cut DNA at specific sites known as restriction sites.
- Restriction sites are palindromic sequences or made up of 4 to 8 nucleotides.
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