DRAW pBR 322 EXPLAIN CHARACTERSTICS OF CLONING VECTOR..
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Characteristics of cloning vector.
1. It should have its own replication point or origin i.e. Ori
2. Ori is a sequence from where replication start on segment of DNA.
3. Ori. helps to support high copy number
4. It must possess a restriction enzymes.
5. It should be in small size.
6. It should be work on prokaryotic as wellas eukaryotic cells.
Here are some features which are required for a cloning vector.
1. Origin of replication.
2. Selectable marker.
3. Cloning sites.
4. Vector for cloning genes in plants and animals as mentioned earlier.
Hope it helps you.
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