Question

DRAW pBR 322 EXPLAIN CHARACTERSTICS OF CLONING VECTOR..​

Answer 1

Hi

here is your answer. .

Characteristics of cloning vector.

1. It should have its own replication point or origin i.e. Ori

2. Ori is a sequence from where replication start on segment of DNA.

3. Ori. helps to support high copy number

4. It must possess a restriction enzymes.

5. It should be in small size.

6. It should be work on prokaryotic as wellas eukaryotic cells.

Here are some features which are required for a cloning vector.

1. Origin of replication.

2. Selectable marker.

3. Cloning sites.

4. Vector for cloning genes in plants and animals as mentioned earlier.

Hope it helps you.

Answer 2

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